بررسی جنین زایی غیرجنسی از تخمک های بارور نشده لیمو آب شیراز(Citrus aurantifolia L. var. Mexican lime) در محیط های کشت مختلف

The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA3 at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, whereas at 1000 mg/l, it inhibited embryogenesis but produced embryogenic callus. Banzyl adenine (BA) at 0.1 and 0.01 mg/l concentrations had significant effects on embryogenesis, while embryo formation was inhibited at high concentrations. Embryo development in different media was also studied. The index of mean root length to mean stem length in the MT3 medium supplemented with 0.1 mg/l of GA3 was observed to be 1.22±0.22, which was regarded a suitable medium for embryo formation, embryo development and plantlet regeneration.